Product Name
GST tag (mouse monoclonal) (clone S-tag-05)
Background
GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Format
Liquid at 1 mg/ml.
Purity
Immunoglobulin purified on Protein A.
Reactivity
GST-tagged proteins
Confirmed reactivity
GST fusion proteins
Tested applications
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 µg/ml (WB)
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.