Product Name
GFP | Green Fluorescence Protein - Biotin conjugated 40 µg
Immunogen
Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
Reactivity
Green Fluorescence Protein (GFP)
Confirmed reactivity
Recombinant GFP overexpressed in E.coli, from: Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum
Recommended dilution
1: 5000 - 1 : 10 000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.