Product Name
His-tag | 6xHis, Biotin Conjugated (Clone AD1,1,10)
Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
Background
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to Biotin. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Purity
Immunogen affinity purified in 50 mM tris (pH 7.8), 0.9% NaCl, 0.05% sodium azide.
Reactivity
Polyhisitidine tagged proteins (N- or C-terminus label)
Reconstitution
For reconstitution add 100 µl of sterile water
Tested applications
Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator
Storage
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.