Product Name
HA (rabbit polyclonal)
Immunogen
KLH-conjugated synthetic peptide: YPYDVPDYA
Background
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Purity
affinity purified serum in PBS, pH 7.4.
Reactivity
Human influenza hemagglutinin (HA) tagged proteins (strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype)
Confirmed reactivity
HA-tag
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 100 µl of sterile water
Expected/apparent MW
Depends on the MW of the protein which is HA-tagged
Tested applications
Western blot (WB), immunoprecipitation (IP)
Recommended dilution
1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.