Product Name
c-Myc tag, Biotin conjugated (mouse monoclonal) (clone 9E10)
Immunogen
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
Background
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Format
Liquid at 1 mg/ml.
Purity
Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin.
Reactivity
c-myc tagged proteins
Confirmed reactivity
c-Myc tagged fusion proteins
Tested applications
Immunoprecipitation (IP), Immunohistochemisty (IHC), paraffin, Flow cytometry (Flowcyt), Western blot (WB)
Recommended dilution
1-5 µg/ml (Flowcyt)
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.