Product Name
Myc tag (monoclonal, Clone Myc,A7)
Immunogen
KLH-conjugated synthetic peptide: EQKLISEEDL (Myc tag)
Background
The anti-Myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc.
Format
Liquid in PBS, pH 7.4, with 0.05% sodium azide
Purity
Protein A purified
Reactivity
Myc-tagged proteins
Confirmed reactivity
Myc epitope tag, fused to N- or C-terminal of proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Tested applications
Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 200 (IL), (IP), 1 : 1000 (WB)
Storage
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes