Product Name
TRITC | tetramethylrhodamine isothiocyanate, HRP-conjugated (monoclonal, Clone NI 308)
Immunogen
Highly purified tetramethylrhodamine isothiocyanate isomer R.
Background
TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.
Purity
Purified mouse IgG.1 kappa in PBS pH 7.2, HRP conjuaged.
Reactivity
TRITC molecule
Confirmed reactivity
Tetramethylrhodamine isothiocyanate isomer R
Reconstitution
For reconstitution add 0.5 ml of sterile water
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
Recommended dilution
The optimum working dilution should be determined by titration by end user. 1:100 (ELISA), 1:10-1:40 (IHC)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
Datasheet 
