Product Name
Fluorescein (monoclonal, clone 6A4)
Background
Fluorescein, a fluorophore is a commonly used dye in microscopy studies.
Purity
Purified on Protein A to a total immunoglobulin fraction in PBS pH 8, sucrose 2%.
Confirmed reactivity
Antibody detects free and bound fluorescein.
Reconstitution
Reconstitute in distilled water before use.
Expected/apparent MW
Depends upon fusion partner
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
1:2000 - 1:20 000 (ELISA), 1: 200 - 1: 2000 (ICC), 1: 500 - 1: 5000 (WB)
Storage
Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.