PsbD | D2 protein of PSII positive control/quantitation standard

PsbD | D2 protein of PSII positive control/quantitation standard

APA-PSQ-005

D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.This product is a recombinant protein standard, source Synechocystis strain PCC 6803.

Lyophilized in glycerol

250 µl

For reconstitution add 225 µl of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

In most gel systems PsbD migrates around 28-30 kDa

Western blot (WB)

Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll will give a PsbD signal in this range. Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems.  This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.