AtpB | Positive control/quantitation standard

AtpB | Positive control/quantitation standard

APA-PSQ-009

ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.

Lyophilized, in glycerol.

100 µl

For reconstitution add 90 µl of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

in most gel systems AtpB migrates around 50-54 kDa

Western blot (WB)

Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a AtpB signal in this range. Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.