PR-1 | Positive control/quantitation standard

PR-1 | Positive control/quantitation standard

APA-PSQ-010

Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.This product is a recombinant PR-1 protein, trunctated by first 26 amino acids, source: Arabidopsis thaliana, UniProt: P33154, TAIR: At2g14610

Lyophilized in glycerol.

100 µl

For reconstitution add 90 µl of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

16.4 kDa

Western blot (WB)

Standard curve: 3 loads are recommended eg.0.5, 2 and 4μl. For most applications a sample load of 10-20 μg of protein will provide with a signal in this range. Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.