Product Name
NifH | Positive control/quantitation standard
Background
Nitrogenase is involved in biological fixation of nitrogen to assimilable ammonia. This product is a recombinant protein standard, source: Nostoc/Anabaena 7120.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Reconstitution
For reconstitution add 90 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Expected/apparent MW
34 kDa (larger than a native protein due to the addition of His-tag)
Tested applications
Western blot (WB)
Recommended dilution
Standard curve: 3 loads are recommended (0.5, 2 and 4 μl).For most applications a sample load of 0.2 μg of chlorophyll will give a NifH signal in this range. Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Datasheet 
