Goat anti-Rat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min,reactivity to human, mouse IgG
Product Name
Goat anti-Rat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min,reactivity to human, mouse IgG
Background
Goat anti-rat IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rat IgGs in immunological assasys. Antibodies are adsorbed against human,mouse IgG and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Purity
Immunogen affinity purified IgG, F(ab)'2 fragment.
Recommended dilution
The optimal working dilution should be determined by the investigator
Storage
Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.