Product Name
Chicken anti-Mouse IgG (H&L), ALP conjugated
Background
Chicken anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Purity
Immunogen affinity purified IgY.
Reactivity
Mouse IgG (H&L)
Recommended dilution
1 : 500-1 : 2000 (WB) 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC),
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.