Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated

Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated

Product Name
Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated
Cat#
APA-GA-084
Host
Goat
Immunogen
purified human IgG (H&L)
Background
Goat anti-human IgG (H&L), F(ab)'2 fragment  is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Format
Lyophilized
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Quantity
0.5 mg
Reactivity
Human IgG (H&L), F(ab)'2 fragment
Confirmed reactivity
Human IgG (H&L), F(ab)'2 fragment
Predicted reactivity
Human IgG (H&L), F(ab)'2 fragment
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 0.55 ml of sterile water
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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