Donkey anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse, rabbit, rat IgG
Product Name
Donkey anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse, rabbit, rat IgG
Background
Donkey anti-goat IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which reacts with all goat IgGs in immunological assays. Antibody is adsorbed to human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Purity
Immunogen affinity purified IgG.
Recommended dilution
1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.