Product Name
Goat anti-Donkey IgG (H&L), HRP conjugated
Immunogen
Purified Donkey IgG, whole molecule.
Background
Goat anti-Donkey IgG (H&L) is a secondary antibody, conjugated to HRP, which binds to Donkey IgG (H&L) in immunological assays.
Purity
Immunogen affinity purified goat IgG.
Reactivity
Donkey IgG (H&L)
Confirmed reactivity
Heavy chains on donkey IgG and light chains on all donkey immunoglobulins
Not reactive in
Non-immunoglobulin donkey serum proteins
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Tested applications
Flow cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 20-1 : 2000 (Flow cyt), (IL), 1: 5000 (WB)
Storage
Store freeze-dried powder at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.