Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, rabbit IgG

Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, rabbit IgG

Product Name
Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, rabbit IgG
Cat#
APA-GA-146
Host
Donkey
Background
Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG.
Format
Liquid
Clonality
Polyclonal
Purity
Immunogen affinity purified IgG.
Quantity
1 mg
Reactivity
Sheep IgG (H&L)
Recommended dilution
1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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