Product Name
14-3-3 GRF | General regulatory element
Immunogen
Conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 “canonical” members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94, TAIR: AT4G09000, GRF2 GF14 omega, UniProt: Q01525, TAIR: AT1G78300, GRF3 GF14 psi UniProt: F4KBI7, TAIR: AT5G38480, GRF4 GF14 phi UniProt: F4HWQ5, TAIR: AT1G35160, GRF5 GF14 upsilon UniProt: P42645, TAIR: AT5G16050, GRF6 GF14 lambda UniProt: P48349#P48349-2, TAIR: AT5G10450, GRF7 GF14 nu UniProt: Q96300, TAIR: AT3G02520, GRF8 GF14 kappa UniProt: F4KHY7, TAIR: AT5G65430, GRF9 GF14 mu UniProt: F4IP55, TAIR:AT2G42590, GRF10 GF14 epsilon UniProt: F4I1C1, TAIR:AT1G22300, GRF11 GF14 omicron UniProt: Q9S9Z8, TAIR: AT1G34760, GRF12 GF14 iota UniProt: Q9C5W6 TAIR: AT1G26480.
Background
GRF (General regulatory factor 2) or 14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.
Reactivity
A. platanoides, A. thaliana, C.australis R.Br., L. longiflorum. M. crystallinum, N. tabacum, O. sativa, P. kurroa, P. vulgaris, algae N. oloabundans, Z.mays
Confirmed reactivity
Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum, Neochloris oloabundans (algae), Oryza sativa, Picrorhiza kurroa, Phaseolus vulgaris, Panax notoginseng, Zea mays, Vicia faba
Predicted reactivity
Brassica sp., Chlamydomonas reinhardtii, Glycine max, Gossypium hirsutum, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Solanum tuberosum, Triticum aestivum
Not reactive in
This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum.
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
20-28 kDa (depending upon an isoform)
Tested applications
Immunoprecipitation (IP), Western Blot (WB)
Recommended dilution
1: 2000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 
