Product Name
KLH | Keyhole limpet hemocyanin, ALP-conjugated (40 µg)
Immunogen
Purified keyhole limpet hemocyanin (KLH), whole molecule.
Background
Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Confirmed reactivity
Megathura crenulata - most commonly used carrier protein
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
ca, 400 kDa/subunit
Tested applications
ELISA (ELISA)
Recommended dilution
1 : 5 000 (ELISA), 1 : 1000 (IL), 1 : 5 000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Datasheet 