Product Name
PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)
Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
Background
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
Reactivity
Global antibody for higher plants, algae, liverwort, cyanobacteria, diatoms
Confirmed reactivity
Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp. , Cannabis sativa L., Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis (Kunth) Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex
Predicted reactivity
Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Cannabis sativa, Galdieria sulphuraria, Lactuca sativa, Lycopersicum esculentum, Medicago sativa, Nannochloropsis sp., Oryza sativa, Ostreococcus sp. Pisum sativum, Sesamum indicum, Thalassiosira pseudonana, Zosteria marina, Vitis vinifera cellular [compartment marker] of thylakoid membrane
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
38 | 28-30 kDa
Tested applications
Immunofluorescence (IF), ImmunoGold (IG), Western blot (WB)
Recommended dilution
1: 500 (IF), 1: 200 (IG), 1 : 10 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 
