Product Name
FtsZ | Procaryotic cell division GTPase (cyanobacterial)
Immunogen
Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein, UniProt: Q3MC27 overexpressed in E.coli.
Background
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Confirmed reactivity
Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Predicted reactivity
Phaeodactylum tricornutum, Prochlorococcus sp.
Not reactive in
higher plants
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
44.5 | 50 kDa
Tested applications
Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.