Product Name
AtpB | Beta subunit of ATP synthase, mitochondrial
Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal mitochondrial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana ATP synthase subunit beta-1 UniProt:P83483, TAIR:AT5G08670ATP synthases subunit beta-2 UniProt:P83484, TAIR: AT5G08690, ATPase subunit beta-3, UniProt: Q9C5A9, TAIR:AT5G08680, which belong to mitochondrial respiratory chain complex I.
Background
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. This enzyme is localised in mitochondrial inner membrane.
Reactivity
Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom’, [compartment marker] of mitochondrial inner membrane
Confirmed reactivity
Arabidopsis thaliana, Brassica oleracea var. botrytis cv. 'Diadom'
Predicted reactivity
Chlamydomonas reinhardtii, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum
Not reactive in
Dunaliella salina
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
59.6 | 55 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.