Product Name
AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl)
Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
Background
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Reactivity
Global antibody for plant, green alga and bacterial F-type ATP synthases
Confirmed reactivity
Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp. (roots, leafs, pollen), Nicotiana bentamiana, Nicotiana tabacum, Populus sp., Spinacia oleracea, Zea mays. Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria
Predicted reactivity
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp.
Not reactive in
Archeal V-type ATP synthase
Reconstitution
For reconstitution add 10 µl of sterile water
Expected/apparent MW
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
Tested applications
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 
