Product Name
AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)
Immunogen
Recombinant maize chloroplast AtpA P05022
Background
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Reactivity
A. thaliana, H. modesticaldum, O. sativa, Z. mays
Confirmed reactivity
Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Oryza sativa, Zea mays
Predicted reactivity
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, Cyanobacteria
Not reactive in
Nicotiana benthamiana
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
55 kDa
Tested applications
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1: 200 (IG), 1 : 5 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.