Immunogen
native bromelain isolated and purified from Ananas comosus
Background
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Purity
Purified IgG in PBS.
Reactivity
Ananas comosus
Confirmed reactivity
Ananas comosus
Predicted reactivity
Ananas comosus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Expected/apparent MW
39 kDa
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.