Product Name
ASN | Glutamine-dependent asparagine synthetase
Immunogen
Purified full length, tag cleaved, recombinant Arabidopsis thaliana ASN2, UniProt: Q9LV77 , TAIR: AT5G65010
Background
ASN (Glutamine-dependent asparagine synthetase) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences of Arabidopsis thaliana ASN1 and ASN2 are 76% identical. The amino acid sequences of Arabidopsis thaliana and Zea mays ASN2 are 73.6% identical.
Format
Liquid at 2 mg/ml.
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Reactivity
Arabidopsis thaliana
Confirmed reactivity
Arabidopsis thaliana, Zea mays
Predicted reactivity
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Punica granatum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
65 | 65 kDa
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
Assay dependent (ELISA), 1: 100-1: 500, paraffin sections (IHC), 1: 1000-1: 2000 (WB)
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.