Product Name
GlnA | Glutamine synthetase
Immunogen
KLH-conjugated synthetic peptide derived from available bacterial GlnA sequences with perfect conservation in alpha, beta, gamma Proteobacteria, Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weak conservation with Trichodesmium thiebautii) including Synechocystis PCC 6803 Q59981
Background
Glutamine synthetase (EC=6.3.1.2) is the key enzyme in the incorporation of mineral nitrogen into glutamine. Activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.
Purity
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02% sodium azide.
Quantity
50 µl (16 mg/ml)
Reactivity
Global antibody for bacteria
Confirmed reactivity
Deinococcus radioduransm Synechococcus sp. strain PCC 7942, Synechocystis sp. strain PCC 6803, Trichodesmium IMS
Predicted reactivity
Alpha, beta, gamma proteobacteria, Arthropsira sp. PCC 8005, Crenarchaeotes, Enterobacteria, Escherichia coli, Euryarchaeotes, Thermotogales
Not reactive in
Diatoms, eukaryotic GlnA
Expected/apparent MW
53 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Storage
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.