Product Name
Glutamine synthetase (leaf,root)
Immunogen
Purified full length, tag cleaved, recombinant Zea mays GS-1 (Glutamine synthetase, root isozyme 1) UniProt: P38559
Background
Glutamine synthetase plays a role in the flow of nitrogen into nitrogenous organic compounds. There are five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.
Format
Liquid at 2 mg/ml.
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Reactivity
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Confirmed reactivity
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Predicted reactivity
Brachypodium distachyon, Cucurbita moschata, Glycine max, Medicago truncatula, Oryza sativa, Panicum hallii, Pontederia crassipes, Populus tremula x Populus alba, Prunus persica, Raphanus sativus, Saccharum officinarum, Setaria italica, Sorghum bicolor, Triticum aestivum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
39 kDa | 43 kDa (chloroplast isoform)
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
Assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.