Product Name
GltBD | NADPH-dependent glutamate synthase
Immunogen
Purified full length, tag cleaved, recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum) , glutamate synthase, UniProt: Q51583 (gltB, large subunit L.boryanum), Q51584 (gltD, small subunit L.boryanum)
Background
NADH-dependent glutamate synthase (GltBD) is involved in glutamate biosynthesis and consists of large subunit (GltB, 168 kDa) and small subunit (GltD, 54 kDa). It is required for non-photorespiratory ammonium assimilation.
Format
Liquid at 4 mg/ml.
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Reactivity
Synechocystis sp. PCC6803
Confirmed reactivity
Synechocystis sp. strain PCC6803
Predicted reactivity
cyanobacteria
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
167.9 | 168 kDa (large subunit)54.2 | 54 kDa (small subunit)
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 1000 - 1: 2000 (WB)
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.