3' Primer
GACAATACAAACTAAGATTTAGTCAG
5' Primer
OpIE2 Forward: CGCAACGATCTGGTAAACAC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, DH5α
Host cells (lines)
Sf9, Sf21, High Five
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Zeocin
Tag
V5 Epitope Tag, His Tag (C-terminal)
Plasmid Type
Insect expression vectors