3' Primer
Polyhedrin Reverse: GTCCAAGTTTCCCTG
5' Primer
Polyhedrin Forward: AAATGATAACCATCTCGC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
E.coli, TOP10
Host cells (lines)
Sf9, Sf21, High Five cell lines
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Viral/Non-Viral
Baculovirus
Plasmid Type
Insect expression vectors, Baculoviral vectors