5' Primer
T7: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Kanamycin
Tag
His (mid and C-terminal), N-Thrombin
Plasmid Type
E. coli protein expression