3' Primer
T7t: GCTAGTTATTGCTCAGCGG
5' Primer
pGEX-5': GGGCTGGCAAGCCACGTTTGGTG
Cloning Method
MCS, Restriction endonucleases
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Kanamycin
Tag
N-GST, N-His, N-Thrombin, N-EK, N-S
Plasmid Type
E. coli protein expression