5' Primer
T7: TAATACGACTCACTATAGGG; pGEX-5': GGGCTGGCAAGCCACGTTTGGTG
Cloning Method
MCS, Restriction endonucleases
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Kanamycin
Tag
N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa
Plasmid Type
E. coli protein expression