Product Name
pET-28a-EGFP
3' Primer
T7-ter: TGCTAGTTATTGCTCAGCGG
5' Primer
T7: TAATACGACTCACTATAGGG
Cloned Strain(s)
DH5α etc.
Induction Method
IPTG or lactose and its analogues
Cultivation condition
37℃, O2, LB
Bacterial Resistance(s)
Kanamycin
Tag
N-6×His, N-Thrombin, N-EGFP, C-6×His
Plasmid Type
E. coli expression plasmids, E. coli fluorescent plasmids, E. coli green fluorescent plasmids