5' Primer
T7 Fwd: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Ampicillin
Tag
His Tag (C-terminal), Strep TagII (N-terminal)
Plasmid Type
E. coli expression vectors, Protein purification