3' Primer
pCold Reverse: GGCAGGGATCTTAGATTCTG
5' Primer
pCold Forward: ACGCCATATCGCCGAAAGG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Constitutive/Inducible
Inducible
Bacterial Resistance(s)
Ampicillin
Tag
GST Tag (N-terminal), HRV 3C protease cut site (N-terminal), His Tag (N-terminal)
Plasmid Type
E. coli expression vectors