5' Primer
T7: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
thioredoxin (N-terminal), His (mid and C-terminal)
Plasmid Type
E. coli protein expression