3' Primer
T7t: TGCTAGTTATTGCTCAGCGG
5' Primer
T7: TAATACGACTCACTATAGGG; Trx-F: TTCCTCGACGCTAACCTG
Cloning Method
MCS, Restriction endonucleases
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Kanamycin
Tag
N-Trx, N-His, N-HRV 3C, C-S, C-Thrombin
Plasmid Type
E. coli protein expression