3' Primer
hGH-pA-R: CCAGCTTGGTTCCCAATAGA
5' Primer
CMV Forward: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Host cells (lines)
Conventional cell lines such as 293, Hela, etc.
Stable
Transient or stable expression
Selectable Marker
Neomycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
FLAG tag (N-terminal)
Plasmid Type
Mammalian expression vectors, Secretory expression vectors