3' Primer
SV40pA rev: GAAATTTGTGATGCTATTGC
5' Primer
CMV fwd: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Mammalian cells
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian expression vectors, cDNA cloning vectors, I.M.A.G.E. vectors