3' Primer
SV40pA rev: GAAATTTGTGATGCTATTGC
5' Primer
CMV fwd: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Host cells (lines)
Mammalian cells
Stable
Transient expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian expression vectors, Cre/loxP vectors, cDNA cloning vectors, In vitro transcription vectors