Product Name
pcDNA6/V5-His B
3' Primer
BGH Reverse: TAGAAGGCACAGTCGAGG
5' Primer
T7 Forward: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10F´, JM109 and INVaF´
Host cells (lines)
Conventional cell lines such as 293, Hela, etc.
Stable
Transient or stable expression
Selectable Marker
Blasticidin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
V5 Epitope (C-terminal), His tag (C-terminal)
Plasmid Type
Mammalian expression vectors, cDNA expression vectors