Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10F′, JM109 and INVaF′
Host cells (lines)
Conventional cell lines such as 293, Hela, etc.
Stable
Transient or stable expression
Selectable Marker
Neomycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Kanamycin
Plasmid Type
Mammalian expression vectors, Gene expression vectors