Product Name
pcDNA5/FRT/V5-His-TOPO
3' Primer
BGH Reverse: TAGAAGGCACAGTCGAGG
5' Primer
T7 Forward: TAATACGACTCACTATAGGG
Cloned Strain(s)
TOP10, DH5α, JM109
Host cells (lines)
293, CV-1, CHO etc.
Stable
Transient or stable expression
Selectable Marker
Hygromycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
V5 Epitope (C-terminal), His tag(C-terminal)
Plasmid Type
Mammalian cell expression vectors, cDNA expression vectors, Targeted reorganization vectors