Terminator
SV40 poly (A) signal
3' Primer
Sv40-polyA-R: GAAATTTGTGATGCTATTGC
5' Primer
CMV-F: CGCAAATGGGCGGTAGGCGTG
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Mammalian cells
Bacterial Resistance(s)
Kanamycin
Plasmid Type
Mammalian cell, Fluorescent protein reporter vectors