Product Name
pcDNA4/myc-His B
3' Primer
BGH Reverse: TAGAAGGCACAGTCGAGG
5' Primer
T7 Forward: TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10F´, DH5a, JM109, TOP10
Host cells (lines)
Conventional cell lines such as 293, Hela, etc.
Stable
Transient or stable expression
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
His Tag (C-terminal), c-Myc Epitope Tag (C-terminal)
Plasmid Type
Mammalian expression vectors, cDNA expression vectors