Terminator
SV40 poly (A) signal
5' Primer
Sv40-polyA-R: GAAATTTGTGATGCTATTGC
Cloned Strain(s)
DH5α etc.
Host cells (lines)
Mammalian cells
Induction Method
Transient expression without induction
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian cell vectors, Signaling pathway reporter vectors