Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
TOP10, DH5α, JM109
Host cells (lines)
293, CV-1, CHO etc.
Stable
Transient or stable expression
Selectable Marker
Hygromycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian expression vectors, cDNA expression vectors, Targeted reorganization vectors